Part:BBa_K530001

CRTE

This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site AgeI at bp 202, making the part compatible with the RFC25 standard. Please see BBa_K2117012 for details of the new and improved part.

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 335
  Illegal XhoI site found at 34
  Illegal XhoI site found at 166
  Illegal XhoI site found at 985
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 202
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization of performance

Below we can see the beta-carotene production levels on a per cell basis for different combinations of parts involved in beta-carotene synthesis. These are compared with a wild type control. tHMG1 is used to funnel more initial substrate into the pathway and although useful, was not submitted as a part. 2I indicates that two copies of crtI were used.

Plasmid Map

Sequencing

This is the sequencing for colony 4.